Quiescent tumor cell models for regulating tumor dormancy

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861735
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kumamoto University (2015); Hamamatsu University School of Medicine (2014)
• Principal Investigator: Shinriki Satoru 熊本大学, 大学院生命科学研究部, 講師 (00583048)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 静止期癌細胞 / 骨髄播種癌細胞 / CYLD / 抗癌剤耐性

Outline of Final Research Achievements

Suppression of CYLD expression by siRNA in head and neck squamous cell carcinoma (HNSCC) cell lines led to an increase in G0 phase. Although, to establish a quiescent tumor cell model, we utilized Tet-on shRNA to regulate CYLD expression, the knockdown efficacy has been insufficient. Therefore, further investigation is needed.
Besides, we established a dormant bone marrow-disseminated tumor cell (BM-DTC)-derived subclone using OSCC cell line, HEp3. Using this subclone, we reported that intrinsic TGF-β2-SDF-1-CXCR4 signaling was important for slow-cycling state and drug resistance in dormant BM-DTC.

Free Research Field

医歯薬学