The influence of immune tolerance by heat shock protein on arteriosclerosis derived from periodontal disease.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26861751
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Nihon University
• Principal Investigator: HAMANO Mio (萩原美緒) 日本大学, 松戸歯学部, 助教 (60724820)
• Research Collaborator: OCHIAI Tomoko ; KOBAYASHI Ryoki
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 熱ショックたんぱく質 / アテローム性動脈硬化症 / 歯周病原菌

Outline of Final Research Achievements

Autoimmune responses to heat-shock protein 60 (HSP60) contribute to the progression of atherosclerosis, whereas immunization with HSP60 may induce atheroprotective responses. We assessed the capacity of an atheroprotective vaccine that targeted a recombinant HSP60 from P. g. (rGroEL) to induce a protective mucosal immune response. Apolipoprotein E-deficient spontaneously hyperlipidemic mice received sublingual delivery of rGroEL prior to P. g. injection. Sublingual immunization with rGroEL induced significant rGroEL-specific serum IgG responses. Furthermore, sublingual immunization with rGroEL significantly reduced atherosclerosis lesion formation in the aortic sinus. These findings suggest that sublingual immunization with rGroEL is associated with the increase of IFN-γ+ or IL-10+ Foxp3+ cells in SMG and a systemic humoral response, which could be an effective strategy for the prevention of naturally occurring or P. g.-accelerated atherosclerosis.

Free Research Field

免疫学