2015 Fiscal Year Final Research Report
Functional salivary gland regeneration using dedifferentiated fat cells
| Project/Area Number |
26861759
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | 再生医療 / 組織工学 / 脱分化脂肪細胞 / 幹細胞 |
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| Outline of Final Research Achievements |
A human buccal fat pad (BFP) was obtained from a patient, who underwent oral and maxillofacial surgery. BFP was dissociated in a collagenase solution. We isolated dedifferentiated fat (DFAT) cells using the "ceiling culture method". This method used the adipocytes' characteristic that they have buoyancy. DFAT cells were passaged and used for flow cytometric analysis. DFAT cells were positive for Stro-1, CD44, CD90, CD105, CD166, and negative for CD19, CD45, CD106, CD146. We also obtained DFAT cells from GFP transgenic mice using the "ceiling culture method". The form of DFAT cells derived from GFP transgenic mice were evaluated using a fluorescence microscope.
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| Free Research Field |
組織工学
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