Research for microRNAs that regulate chondrogenesis in condylar cartilage under mechanical stress

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861792
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Kyushu University
• Principal Investigator: Hoshi Kenji 九州大学, 大学病院, 助教 (90569964)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 軟骨細胞 / メカニカルストレス / microRNA

Outline of Final Research Achievements

We examined the chondrogenesis of mesenchymal stem cells under mechanical stress, focusing on micro RNAs (miRNA). Mesenchymal stem cells derived from fetal mouse condylar cartilages were seeded on a culture device that can apply tensile stress. We loaded tensile stress for 3 hours, 1 day and 2 days. After the application of tensile stress, microarray analysis was performed to detect miRNAs whose expression levels were characteristically changed. We also examined whether the detected miRNAs target chondrogenic marker genes. According to this analysis, the expression levels of miR-134-5p, 152-3p, 29a-3p, 431-5p, 145a-5p, 342-3p and 125a-3p were increased in loaded groups compared with control groups. These miRNAs were found to target Col2a or Sox9.

Free Research Field

歯科矯正学