Development of surface modification techniques for coherent X-ray diffraction imaging of in-solution biological samples.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870006
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Quantum beam science; Nanobioscience
• Research Institution: Hokkaido University
• Principal Investigator: Kimura Takashi 北海道大学, 電子科学研究所, 助教 (50531472)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: X線光学 / X線イメージング / 超精密加工 / X線自由電子レーザー / コヒーレント回折イメージング

Outline of Final Research Achievements

In this research, I developed surface modification techniques in order to advance the potential of in-solution biological sample imaging by X-ray free-electron laser coherent diffraction microscopy. The surface modification technique we developed includes the fabrication of 30-nm-damenter dot pattern with electron beam lithography on 200-nm-thickness silicon nitride membrane which is used as sample holder for coherent diffraction microscopy. On each dot pattern, we successfully mounted protein molecules with the assistance of self-assembled monolayer. Coherent diffraction patterns from such uniquely fabricated samples were obtained at Japanese X-ray free-electron laser facility SACLA (SPring-8 Angstrom Compact free-electron LAser) and the image was reconstructed by using phase retrieval algorithms.

Free Research Field

X線イメージング