Study on role of the placenta-specific gene to imprint in the nuclear initialization

2016 Fiscal Year Final Research Report

• Project/Area Number: 26870036
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology; Obstetrics and gynecology
• Research Institution: Tohoku University
• Principal Investigator: Chiba Hatune 東北大学, 医学系研究科, 助教 (80642719)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ゲノムインプリンティング / メチル化インプリント / クローンマウス / 胎盤

Outline of Final Research Achievements

Both Gab1 and Sfmbt2 are novel placenta-specific imprinted genes and we analyzed the function and regulatory mechanism of Gab1 and Sfmbt2 using Gab1 KO mice, trophoblast stem cells and cloned mice. We found that the paternal expression of Gab1 and Sfmbt2 were regulated by a novel differentially methylated region (DMR) and the imprinted expression was important for normal placental development. We also found that in cloned mouse placentas, the Gab1 and Sfmbt2 DMR were lost and Gab1 and Sfmbt2 were highly expressed, which may contribute to placentomegaly of cloned mice.

Free Research Field

分子生物学