2015 Fiscal Year Final Research Report
Fabrication of 3D perfusable macroscopic tissue with hierarchical structures
| Project/Area Number |
26870137
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nano/Microsystems
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 3次元組織構築 / 組織工学 / バイオマテリアル / マイクロ流体デバイス |
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| Outline of Final Research Achievements |
In this research, fabrication technique of three-dimensional perfusable macroscopic tissues using cell fibers was described. Various cells were able to be encapsulated in the fiber-shaped hydrogel, namely a cell fiber, using microfluidic device. It was succeeded to rapidly create hierarchical tissues using a method to reel cell fibers around degradable hydrogel supports. After the creation of hierarchical tissues, decomposition and removal of hydrogel support using with enzyme enable to fabricate luminal tissue structures that was able to be perfused with culture medium using a peristaltic pump. Comparing hierarchical tissue of hepatoma and fibroblast with monolayer tissue of hepatoma, it was observed that the rate of albumin secretion of a hierarchical tissue was higher than that of a monolayer tissue. We have prepared to submit the paper about this research.
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| Free Research Field |
組織工学、再生医療、バイオマテリアル
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