Analysis of expression profile of cell surface proteins, and establishment of specific cell adhesive materials for differentiation of human iPS cells into hepatic lineage cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870230
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology; Biomedical engineering/Biomaterial science and engineering
• Research Institution: University of Fukui
• Principal Investigator: NAGAOKA Masato 福井大学, テニュアトラック推進本部, 助教 (90397050)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ヒトiPS細胞 / 接着基質 / バイオマテリアル / 分化誘導 / 肝細胞

Outline of Final Research Achievements

In this study, defined cell-recognizable materials have been developed for the differentiation of human iPS cells into hepatocyte-like cells. In most of protocols reported previously, Matrigel is used as a substrate for cell adhesion during differentiation. Matrigel is derived from mouse sarcoma, and the method using Matrigel is not a defined condition. First, a small fragment of vitronectin containing RGD sequence was fused with Fc domain of IgG (R-Fc) to establish a defined substrate. This protein supported both maintenance of the pluripotent state of iPS cells and differentiation into hepatocyte-like cells. Next, the patterns of cell-surface protein expression during differentiation were analyzed by DNA array and qPCR to design novel cell adhesive materials for differentiated cells, and specific genes were identified, which expression were up-regulated during differentiation of human iPS cells into hepatic lineage cells.

Free Research Field

細胞生物学