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2015 Fiscal Year Final Research Report

Role of RNA methylation in the regulation of gene expression.

Research Project

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Project/Area Number 26870283
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Environmental physiology(including physical medicine and nutritional physiology)
Biological pharmacy
Research InstitutionKyoto University

Principal Investigator

Fustin Jean-Michel  京都大学, 薬学研究科(研究院), 講師 (50711818)

Project Period (FY) 2014-04-01 – 2016-03-31
Keywordsm6A / circadian
Outline of Final Research Achievements

This proposal had two parts: 1, investigate circadian variations in the m6A methylation of messenger RNA; 2, investigate the roles of Fto, Alkbh5 and Mettl3 in the regulation of gene expression.
Our results for the first part have yielded significant insights on the link between the circadian clock and RNA methylation. We have identified transcripts that are highly methylated across the circadian cycles and that are currently investigated. Moreover, we have identified a significant group of transcripts whose methylation show circadian rhythms of m6A methylation, and are currently preparing a manuscript.For the second part, both Fto and Alkbh5 knock-out mice gave negative results, and no circadian phenotypes were detected. Therefore, we are currently breeding double knock-out mice. Mettl3 knock-out mice were embryonic lethal and conditional knock-out mice have been generated and are currently being mated with SCN-CRE and Alb-CRE to generate SCN and liver-specific KO mice.

Free Research Field

systems biology

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Published: 2017-05-10  

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