2016 Fiscal Year Final Research Report
Functional regulation of cytoskeletal organization by a highly insoluble actin bundling protein Caprice
| Project/Area Number |
26870302
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
Cell biology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 細胞骨格 / 生化学 / 細胞生物学 / アクチン |
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| Outline of Final Research Achievements |
The novel actin-bundling protein Caprice is characteristic in its 1) highly insoluble nature, 2) drastic effect on cellular morphology by over expression or knockdown, and 3) cell type-specific expression. We demonstrated that high concentration of Caprice protein in solution make it into a hydrogel, thus this protein is expected to confer specific property on the actin filament by undergoing sol-to-gel phase transition. Detailed analyses focusing on its transcriptional control and knock-down effect on cellular activities such as morphological regulation and adhesion properties reveled important roles of Caprice in organizing cytoskeletal dynamics.
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| Free Research Field |
生化学、細胞生物学、分子生物学
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