2016 Fiscal Year Final Research Report
Analysis of mechanism by which tetrandrine modulates lipid degradation via blockade of autophagy
| Project/Area Number |
26870309
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioorganic chemistry
Biomolecular chemistry
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| Research Institution | University of Tsukuba (2016)
Kyoto University (2014-2015) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | オートファジー / 脂肪滴 / テトランドリン / perilipin / 肝線維化 |
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| Outline of Final Research Achievements |
Autophagy is a cellular quality control system which degrades unnecessary proteins and organelles. In this study, we analyzed the mechanism by which tetrandrine, a plant-derived isoquinoline alkaloid, modulates lipid degradation via blockade of autophagy. We showed that the compound inhibits the autophagy pathway without affecting lysosomal function. A phenotypic comparison using siRNA knockdown suggested that tetrandrine may target regulators, involved in fusion between autophagosomes and lysosomes. Moreover, perilipin, an lipid droplet (LD)-coated protein, co-localized specifically with LC3, a marker protein for autophagosomes, in tetrandrine-treated cells. This suggests a potential role for perilipin in autophagy-mediated LD degradation.
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| Free Research Field |
生物分子化学
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