Construction of versatile flocculation system and its application for two-stage production system

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870363
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology; Biofunction/Bioprocess
• Research Institution: Kobe University
• Principal Investigator: Tsuge Yota 神戸大学, 自然科学系先端融合研究環, 特命助教 (00647422)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: コリネ型細菌 / 有機酸 / アミノ酸 / フルフラール / 発酵

Outline of Final Research Achievements

We firstly investigated cell growth of amino acid producing Corynebacterium glutamicum under furfural stress. The growth was inhibited by furfural, but furfural was degraded into furfuryl alcohol and 2-furoic acid overtime. Through constructing deletion strains of alcohol dehydrogenase genes, we identified the gene responsible for the conversion of furfural into furfuryl alcohol. Purified protein of this gene converted furfural into furfuryl alcohol in vitro using NADPH as a co-factor.
We moreover constructed a strain by metabolic engineering producing amino acid lysine under aerobic conditions and organic acid succinic acid under anaerobic conditions using oxygen as a metabolic switch.

Free Research Field

Applied Microbiology