Roles of macrophages in early esophageal carcinogenesis: analysis of cell-to-cell interaction between esophageal epithelial cells and macrophages

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870364
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology; Human pathology
• Research Institution: Kobe University
• Principal Investigator: Koma Yuichiro 神戸大学, 医学(系)研究科(研究院), 講師 (40714647)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 腫瘍関連マクロファージ / 食道扁平上皮癌 / 食道上皮内腫瘍 / p38 MAPキナーゼ / インターロイキン6

Outline of Final Research Achievements

In this study, we established coculture assay system using immortalized human esophageal epithelial cells (Het-1A) and human acute monocytic leukemia cells (THP-1)-derived macrophages to study the roles of macrophages in esophageal carcinogenesis. Coculture of Het-1A cells with THP-1-derived macrophages induced Het-1A cell proliferation/migration and phosphorylation of p38 MAP kinase. In addition, IL-6 was significantly increased in coculture supernatants. Finally, positive immunoreactivity for phospho-p38 MAP kinase and IL-6 in neoplastic cells were observed in some cases of esophageal high grade intraepithelial neoplasias.

Free Research Field

医歯薬学