2016 Fiscal Year Final Research Report
Effect of postpartum resumption of ovarian activity on the formation of antral follicle-like structures by bovine cumulus-oocyte complexes in Holstein cows.
| Project/Area Number |
26870406
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
Veterinary medical science
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ウシ体外卵胞発育モデル / 分娩後卵胞波再帰 / セリシン / 黄体形成ホルモン |
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| Outline of Final Research Achievements |
This study investigated the effects of postpartum resumption of ovarian activity on the formation of antral follicle-like structures (AFLS). The results indicate that 1) supplementation of F/P MPs in collagen gels contributed to the beneficial effects of follicle stimulating hormone (FSH) on the formation of antral follicle-like structures. 2) Supplementation with 4% sericin cumulus-oocyte complexes (COCs) formed AFLS and the same formation rates as the supplementation of FBS. 3) Supplementation with serum from postpartum Holstein cows, it is necessary to supplement FSH. Moreover, 4) supplementation with hCG was detrimental. In conclusion, culture techniques of AFLS derived from COCs might provide important insights into follicular development, and Luteinizing hormone may be harmful to a postpartum resumption of ovarian activity. Although improvement of the AFLS culture system is necessary, this system might become a technique to investigate postpartum resumption of ovarian activity.
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| Free Research Field |
獣医臨床繁殖学
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