2015 Fiscal Year Final Research Report
Analysis of mechanisms of wing development in hemimetabolous insect using conditional knock-out method.
| Project/Area Number |
26870415
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Insect science
Developmental biology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | ゲノム編集 / ノックイン / ノックアウト |
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| Outline of Final Research Achievements |
We attempted to establish the knock-in experiment mediated non-homorogous end joining repair pathway in the two spotted cricket. As a result, for the first time in the cricket, we succeeded to generate some knock-in lines inserted the expression cassette of marker gene. For conditional knock-out experiment, we constructed the knock-in vector containing component of piggybac system, and introduce into cricket genome. Furthermore, genome sequence analysis was performed by next generation sequencer PacBio RSII. We obtained about 5Gb data as long read sequence, and assemble to cricket genome sequence for Gap-closing analysis.
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| Free Research Field |
分子生物学
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