Quantitative modeling and systemic understanding of the regulatory mechanisms of the E. coli ammonium assimilation

2016 Fiscal Year Final Research Report

• Project/Area Number: 26870432
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: System genome science; Life / Health / Medical informatics
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: Maeda Kazuhiro 九州工業大学, 若手研究者フロンティア研究アカデミー, 特任助教 (50631230)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 大腸菌 / 窒素代謝 / 生化学パラメータ推定 / シミュレーション

Outline of Final Research Achievements

For E. coli, glutamate and glutamine are important as precursors for amino acids and nucleotides, and ammonium is necessary for the synthesis of glutamate and glutamine. Construction of a mathematical model is indispensable for understanding the complex control mechanism of ammonium assimilation. In this study, we constructed a quantitative dynamic model of the E. coli ammonium assimilation network. We pointed out that AmtB, which was considered to be an ammonia channel so far, is an active ammonium transporter. In addition, we developed a novel kinetic parameter estimation method to accelerate the kinetic modeling and developed a C language library to support it.

Free Research Field

システム生物学