2015 Fiscal Year Final Research Report
The establishment of the model of radiation-induced thyroid cancer and analysis of the inhibitory effect of an antioxidant on DNA damage in thyroid.
Project/Area Number |
26870440
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Endocrinology
Risk sciences of radiation and chemicals
|
Research Institution | Nagasaki University |
Principal Investigator |
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Keywords | DNA二重鎖切断 / 抗酸化剤 / comet assay / 放射線 / 内部被ばく |
Outline of Final Research Achievements |
To establish the in vitro model of the external irradiation with X-ray or internal irradiation with 131-I for analysis of radiation-induced DNA damage response, DNA double strand breaks (DSBs), as an index for DNA damages, were quantified following external or internal irradiation using the neutral comet assay in a normal rat thyroid cell line PCCL3. Then by using this new model established, the inhibitory effect of an anti-oxidant NAC on DNA damages was evaluated. In internal irradiation, administration of NAC even after addition of 131-I was effective at suppressing DSBs. However, this type of in vivo model was failed to be established, and mouse model of radiation-induced thyroid cancer using genetically modified mice showed no histological alterations.
|
Free Research Field |
内分泌学
|