Analysis for mobility and molecular mechanism of centromeric non-coding RNP in chromosome segregation

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870442
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology; Genetics/Chromosome dynamics
• Research Institution: Kumamoto University
• Principal Investigator: Ideue Takashi 熊本大学, 自然科学研究科, 助教 (20420250)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ノンコーディングRNA / セントロメア / 染色体分離 / RNP / 抗がん戦略

Outline of Final Research Achievements

Non-coding (nc)RNA transcribed from human centromere region forms RNA-Protein complex with several factors, and have a role in chromosome segregation and cytokinesis. In this study, I have identified several factors associate with centromere ncRNA and analyzed these functions in chromosome segregation and cytokinesis as a components of centromere ncRNP.
RBMX, IMP-3, IQGAP1 are identified as a components of centromeric ncRNP. IP experiment revealed that these factors associates with centromere ncRNA. Depletion of RBMX showed abnormal chromosome segregation and sister chromatid separation.IMP-3, IQGAP1 and Centromere ncRNA shows localization of central spindle and midbody which concern to cytokinesis after chromosome segregation. Depletion of ncRNA and these factors show abnormal cytokinesis in addition of defects of chromosome segregation.These results reveals that these factors have a role in not only chromosome segregation but also cytokinesis together with centromeric ncRNA.

Free Research Field

分子生物学