Lipid production by oleaginous yeast and biodiesel fuel production by cell surface displayed lipase

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870504
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology; Biofunction/Bioprocess
• Research Institution: Osaka Prefecture University
• Principal Investigator: YAMADA Ryosuke 大阪府立大学, 工学(系)研究科(研究院), 助教 (40608626)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 酵母 / バイオディーゼル燃料 / リパーゼ / 細胞表層提示

Outline of Final Research Achievements

Dwindling petroleum supplies and growing environmental concerns over petroleum use have led to increasing interest in using biodiesel fuel for transportation. In this study, we aimed to construct novel biodiesel production strategy comprised lipid production by oleaginous yeast and esterification of produced lipid by lipase expressed on yeast cell surface without addition of lipase. As a result, lipid production was improved by UV-mutagenesis, cell surface lipase activity was improved by combinatorial library strategy, and biodiesel fuel was successfully produced by lipase displayed on yeast cell surface.

Free Research Field

生物化学工学