Analyses of candidate genes with multi-step DNA methylation alterations in lung cancer

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870569
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology; Respiratory organ internal medicine
• Research Institution: Keio University
• Principal Investigator: Sato Takashi 慶應義塾大学, 医学部, 共同研究員 (20464836)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 肺がん / DNAメチル化 / エピジェネティクス

Outline of Final Research Achievements

Lung cancer is the leading cause of cancer-related death worldwide. It is known that epigenetic changes contribute to human carcinogenesis. We examined candidate genes which were obtained from genome-wide DNA methylation analysis. mRNA expression of PTPRH, one of the candidate genes, were upregulated in non-small cell lung cancer tissues compared with non-cancerous lung tissues and inversly correlated with DNA methylation levels in a cohort of lung adenocarcinoma patients. DNA-demethylating treatment of cell lines restored mRNA
PTPRH expression levels, suggesting that PTPRH gene expression was regulated by DNA hypomethylation. Furthermore, we identified the DNA methylation status of PTPRH as a novel prognostic factor for lung adenocarcinoma.

Free Research Field

医歯薬学