Artificially Controlled Replication Machinery for Bacterial Genome Design

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870575
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: System genome science; Genome biology
• Research Institution: Keio University (2015); Osaka University (2014)
• Principal Investigator: KONO Nobuaki 慶應義塾大学, 政策・メディア研究科, 特任助教 (90647356)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 合成生物学 / バイオインフォマティクス / ゲノム複製 / ゲノム構造 / 枯草菌

Outline of Final Research Achievements

In this project, about a bacteria harboring circular chromosome, we set a goal to recognize the relationship between the genomic structure and replication efficiency, and design a bacterial genome by using various approaches in bioinformatics and genetics. As the results, first, we succeeded in constructing various mutants whose genomic structures were disrupted by inversion and dissection mutations in Bacillus subtilis, and developed a protocol for replication profiling method to observe the behavior of replication forks in cell. Using the mutants and methods, we cleared that the treatment of some gene factor enables to control the replication machinery.

Free Research Field

合成生物学