Establishing an advanced cell-base assay to evaluate the safety of resin monomers

2016 Fiscal Year Final Research Report

• Project/Area Number: 26870678
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Prosthodontics/ Dental materials science and; Functional basic dentistry
• Research Institution: Tohoku University
• Principal Investigator: Orimoto Ai 東北大学, 大学病院, 医員 (30710967)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ARE活性化 / ルシフェラーゼ / レジンモノマー / 歯科材料 / 細胞毒性

Outline of Final Research Achievements

The anti-oxidant responsive element (ARE)-mediated transcription is a key event in cellular detoxification of electrophilic exnobiotics. We previously established a clonal cell line stably transfected with an ARE-destabilized luciferase reporter gene, and showed that 2-hydroxyethyl methacrylate (HEMA), which is more toxic than methyl methacrylate (MMA), highly increased ARE-mediated trancriptional activity at low concentrations than MMA. We here showed the dose-dependent effects of ethyl methacrylate (EMA) on ARE activity and their relation to cytotoxicity. These results showed that the low concentration effects of resin monomers on ARE activity reflect their cytotoxicity probably due to their electroreactivity to intracellular molecules.

Free Research Field

歯科保存