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2015 Fiscal Year Final Research Report

Expression and functional analyses of Crb3a in tumor tissues

Research Project

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Project/Area Number 26870692
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Tumor biology
Experimental pathology
Research InstitutionNiigata University (2015)
Aichi Medical University (2014)

Principal Investigator

Iioka Hidekazu  新潟大学, 医歯学系, 助教 (20425416)

Project Period (FY) 2014-04-01 – 2016-03-31
Keywords細胞極性 / 腫瘍 / 浸潤 / 転移 / ガングリオシド / シアリダーゼ / ノイラミニダーゼ
Outline of Final Research Achievements

The function of Crumbs3 (Crb3)in human tumorigenesis is not fully understood. Western blots and immunohistochemistry revealed that Crb3 is strongly expressed in a part of human adenocarcinoma cell lines, and is localized in the luminal plasma membrane not only in normal tissue but also in tubular cancers. Crb3 knockout (KO) colon cancer cell lines are established by CRISPR/CAS9 system, and Crb3 KO cells showed remarkable reduction in cell migration in vitro and in vivo. Ganglioside has been shown previously to suppress tumorigenesis by inhibiting plasma membrane receptors, and we found that ganglioside was increased on plasma membrane in Crb3 KO cells. Restoration of Crb3 in KO cell blocked plasma membrane localization of ganglioside. These data indicate that Crb3 suppresses ganglioside localization to plasma membrane, to inhibit tumor cell behaviors.

Free Research Field

腫瘍病理学

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Published: 2017-05-10  

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