Identification of the origin of breast cancer stem cells thorough live cell imaging of the mice for visualization of mammary stem cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26870729
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery; Tumor biology
• Research Institution: Kansai Medical University
• Principal Investigator: ATSUMI Naho 関西医科大学, 医学部, 助教 (90612151)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 乳腺幹細胞 / がん幹細胞 / 細胞系譜追跡

Outline of Final Research Achievements

The cell population containing mammary stem cells, which reconstitutes the whole mammary tissues when allotransplanted into fat pad, was identified by Shackleton et al. and Stingl et al. in 2006. However, it was largely unknown about the localization of mammary stem cells due to absence of specific stem cell marker. Here we developed the methodology for the identification of mammary stem cell marker by multicolor lineage tracing using Rosa26-rainbow mice we previously developed. In addition, combining with model mice for breast cancer, we found the candidate markers for breast cancer stem cells. This study will lead to the elucidation of the mechanism for breast cancer development thorough identification of the origin of breast cancer stem cells.

Free Research Field

幹細胞生物学、分子細胞生物学、腫瘍細胞生物学