Development of marine diatom cells which complexly have the ability for high-efficiently producing useful substances

2016 Fiscal Year Final Research Report

• Project/Area Number: 26870750
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology; Environmental engineering and reduction of environmental burden
• Research Institution: Kwansei Gakuin University
• Principal Investigator: Nakajima Kensuke 関西学院大学, 理工学研究科, 博士研究員 (50709318)
• Research Collaborator: SUGIYAMA Toshiki ; FUKUCHI Youhei ; KIMURA Nanae
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 海洋性珪藻 / 無機炭素獲得 / リン酸獲得 / 輸送体 / 有用物質生産

Outline of Final Research Achievements

Marine diatoms are responsible for global primary productivity and play a key role in global cycle of phosphate. Dissolved inorganic carbon and phosphate acquired from seawater are used for producing useful substances like oils and fats, and polyphosphates. In this study, we attempted to identify the specific transporters involved in mechanisms of inorganic carbon and phosphate acquisitions, and develop marine diatom cells which have the ability for high-efficient producing useful substances by co-overexpressing inorganic carbon and phosphate transporters. As a result, we succeed to characterize the chloroplastic membrane-type inorganic carbon transporter, PtSLC4-7, and the plasma membrane-type phosphate transporter, PtSLC34-2. Then, some polyphosphate synthase and degrading enzyme candidates were found on the genome of marine diatoms, and new transformation vector for marine diatom cells was constructed for co-overexpressing two genes driven by conditional promoters.

Free Research Field

植物分子生理学