2016 Fiscal Year Final Research Report
The effect of therapeutic angiogenesis using by CD11b+ cells transplantation in a murine model of hind limb ischemia
| Project/Area Number |
26870785
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Cardiovascular medicine
Cardiovascular surgery
|
|---|
| Research Institution | Fukuoka University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 虚血肢 / マクロファージ / 血管再生 |
|---|
| Outline of Final Research Achievements |
The use of cultured BMCs can reduce the total number of injections required and were shown to induce therapeutic angiogenesis in a murine model of hind limb ischemia. Angiogenesis, lymphangiogenesis, and blood flow recovery ratio were significantly higher in the GM-CSF-cultured F4/80+ macrophage (GM-Macrophage)-treated group compared with controls. Furthermore, Foxp3+ cell numbers and tissue IL-10 concentrations were significantly increased compared with controls. There was no significant difference in blood flow recovery between GM-Macrophage and M-CSF-cultured F4/80+ macrophages (M-Macrophage). Thus, GM-Macrophage were associated with improved blood flow in hind limb ischemia similar to M-Macrophage. The selective methods of culturing and treating GM-Macrophage cells similar to M-Macrophage cells could be used clinically as a novel cell therapy for critical limb ischemia.
|
| Free Research Field |
細胞治療
|
