Development of novel protein stabilization method with multiple site-selective nonnatural amino acids incorporation

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870853
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry; Biomolecular chemistry
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Ohtake Kazumasa 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, 特別研究員 (80593631)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 応用生物化学 / 酵素化学 / 非天然型アミノ酸 / タンパク質工学 / 生体分子の化学修飾 / バイオテクノロジー / タンパク質の安定化 / 拡張遺伝暗号

Outline of Final Research Achievements

Ｉ developed novel protein stabilization method based on multiple incorporation of halogen atoms into tyrosine sites. For the production of such stabilized proteins, multiple site-selective incorporation of halogenated tyrosines is needed. The method was realized by our original technology, genetic code reassigned Escherichia coli strain. Stabilization manner was revealed by X-ray crystallographic analysis and quantum-chemical calculations technique. Then, application possibility to other proteins was suggested by the analysis of existing 3D structures.

Free Research Field

分子生物学 生化学 タンパク質科学