Establishment of a method for the determination of boron in blood samples and its application to a pharmacokinetic study.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26870866
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor therapeutics; Medical engineering assessment
• Research Institution: Japan Chemical Analysis Center
• Principal Investigator: Yamaguchi Yurie 公益財団法人日本分析センター, その他部局等, 研究員(移行) (30639977)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: BNCT / Boron / ICP-MS / BPA / BSH

Outline of Final Research Achievements

To make BNCT treatment safe and effective, precise and accurate determination of B-10 level in blood as well as accurate neutron dosimetry is required for proper control of neutron irradiation conditions. In this study, a method was developed to determine total B and B-10 content in whole blood samples based on inductively coupled plasma mass spectrometry (ICP-MS) which makes it possible to determine the individual isotopes of each element.Then the method was applied to rat blood fractions collected serially after the administration of either BPA or BSH alone or in combination, and the distribution between rat blood cells and plasma was derived as a function of time. The results indicate that the distribution of B-10 for BPA is different from that for BSH. Further evaluation in different administration conditions will be made to clarify the behavior of the two boron compounds in blood.

Free Research Field

分析化学