Determination of the role of Fcgamma receptor in antibody-dependent enhancement of dengue virus infection

2016 Fiscal Year Final Research Report

• Project/Area Number: 26870872
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology; Virology
• Research Institution: Nagasaki University
• Principal Investigator: MOI Meng Ling 長崎大学, 熱帯医学研究所, 准教授 (40597499)
• Research Collaborator: KURANE Ichiro ; TAKASAKI Tomohiko ; MORITA Kouichi
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: デング熱 / 抗体依存感染増強 / Fc gamma receptor

Outline of Final Research Achievements

In this research we aim to determine the role of Fcgamma receptor in infection-enhancement (ADE) of dengue virus (DENV). Here, we have constructed the plasmids of Fcgamma receptors (I, IIA, IIB, III), and expressed the plasmids into DENV-permissive cells to establish cell lines that stably express each of the receptors. Using these cell lines, DENV infectivity was examined using conventional plaque assay and real-time PCR assay. We then determined the ADE activity to DENV using monoclonal antibodies and patient serum samples using the Fcgamma BHK cell lines. Using these monoclonal antibodies, we determined DENV infectivity in cell lines. The ADE could be detected in cell lines that express the Fcgamma IIA and IIB receptor. Overall, we have established Fcgamma receptor expressing cell lines that are useful in DENV diagnosis and pathogenesis studies.

Free Research Field

ウイルス学