Generation of transgenic monkey with tetracyclin-inducible gene expression system for neurodegenerative disease model.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26870884
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science; Nerve anatomy/Neuropathology
• Research Institution: Shinshu University
• Principal Investigator: TOMIOKA Ikuo 信州大学, 学術研究院農学系, 助教 (30528196)
• Co-Investigator(Renkei-kenkyūsha): NAGAI Yoshitaka 大阪大学, 医学部, 教授 (60335354) ; SEKI Kazuhiko 国立精神・神経医療研究センター, 部長 (00226630)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: マーモセット / トランスジェニック / TETシステム / 遺伝子改変 / 疾患モデル / 神経変性疾患

Outline of Final Research Achievements

Artificially controllable transgene expression system is indispensable tool for production of various disease model animals to precisely mimic human disease states. In the present study, we successfully generated transgenic marmoset line with tetracyclin-inducible gene expression system for neurodegenerative disease model. Mutant human ataxin 3 gene with 120 CAG repeats controlled by tetracycline-inducible system was introduced into marmoset embryos via lentiviral transduction. A total of seven live offspring were obtained. They and their fibroblast cells treated doxycycline revealed inducible transgene expression compared to before treatment. Moreover, a total of five second-generation (F1) offspring were obtained from founder marmosets and one was transgenic, showing successful germline transmission of transgene. In conclusion, the present study generated transgenic marmoset line having mutant human ataxin 3 gene controlled by tetracycline-inducible system.

Free Research Field

発生工学