The functional analysis of transcription factors and transcriptional co-factors related to embryonic female mouse gonadal differentiation

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870888
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology; System genome science
• Research Institution: National Center for Child Health and Development
• Principal Investigator: Kato Tomoko 国立研究開発法人国立成育医療研究センター, 研究所 システム発生・再生医学研究部, 研究員 (10638802)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 卵巣 / 転写因子 / 性分化 / ノックアウトマウス

Outline of Final Research Achievements

I performed the expression and functional analysis of 18 ovarian differentiation candidate genes to reveal transcriptional network during ovarian differentiation. To elucidate the transcriptional interaction among these genes, first, luciferase assay using cultured cell based overexpression system was conducted. Second, high-throughput screening using same system was conducted to find the novel candidate genes. In addition, in order to identify the essential genes for female gonadal differentiation in vivo, knockout mice for six genes among them were generated using genome-editing technology, CRISPR/Cas9. However, no gonadal abnormality was detected so far.

Free Research Field

性分化