Transcription factor complex formation and gene expression control in energy metabolism

2015 Fiscal Year Final Research Report

• Project/Area Number: 26882008
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied health science
• Research Institution: University of Tsukuba
• Principal Investigator: HAN SONGIEE 筑波大学, 医学医療系, 研究員 (80729541)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: CREBH

Outline of Final Research Achievements

CREBH is a transcription factor, which is expressed in liver and intestine. CREBH regulates whole-body lipid metabolism. CREBH can bind to PPARα and its co-activator PGC-1α, leading to activation of FGF21 expression. CREBH also binds to SREBP, a master regulator for fatty acid and cholesterol synthesis. This association suppressed the transcriptional activity of SREBP. We identify that the cross-talk among CREB3L3 and transcription factors maintains energy homeostasis and improves metabolic syndrome. We developed liver-specific CREBH KO mice using one-step CRISSPR/Cas9 system. We find the deficiency of CREBH in liver leads to non-alcoholic fatty liver disease.

Free Research Field

分子生物学