2015 Fiscal Year Final Research Report
In-situ observation of protein crystallization by fluid reaction-transmission electron microscopy (FR-TEM)
| Project/Area Number |
26887001
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Condensed matter physics I
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| Research Institution | Hokkaido University |
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Principal Investigator |
YAMAZAKI Tomoya 北海道大学, 低温科学研究所, 学術研究員 (50735032)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Keywords | 透過型電子顕微鏡 / リゾチーム / 結晶化 / 核生成 / 結晶成長 / 溶液成長 / “その場”観察 / タンパク質 |
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| Outline of Final Research Achievements |
The fluid reaction transmission electron microscopy (FR-TEM), which can directly observe inside liquids at nano-scale and in real time, was applied to crystallization of lysozyme protein in a solution.
By optimizing the crystallization condition, in-situ observation of crystallization of lysozyme was successfully performed. Two crystalline phases (orthorhombic and tetragonal) and an amorphous phase coexisted at nano-scale under the solution condition. The moment of the nucleation of orthorhombic lysozyme crystal was successfully captured.
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| Free Research Field |
結晶成長学
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