Development of Quenchbody to image and quantify tumor-associated membrane protein claudin

2015 Fiscal Year Final Research Report

• Project/Area Number: 26889028
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Jeong Hee-Jin 東京工業大学, 資源化学研究所, 助教 (70737981)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: クエンチボディー / クローディン / 免疫センサー / 細胞イメージング

Outline of Final Research Achievements

In this study, we successfully detected claudins which are abnormally expressed on cancer cells by Quenchbody(Q-body)-based simple and convenient novel immunoassay method. We constructed Q-body using anti-claudin antibody gene and measured the fluorescent intensity in the present of purified claudin. As a result, the intensity was claudin-concentration dependently increased and showed the maximum 3.1-fold response. Moreover, when we added the Q-body into the claudin-expressed cells, it showed significant fluorescent, indicating the effort of this Q-body for imaging the over-expressed claudins.

Free Research Field

抗体工学