2015 Fiscal Year Annual Research Report

睡眠の「深さ」の制御機構－徐波睡眠調節を担う皮質ネットワークの解明

Research Project

Project/Area Number	26890003
Research Institution	University of Tsukuba
Principal Investigator	Vogt Kaspar 筑波大学, 国際統合睡眠医科学研究機構, 准教授 (80740034)
Project Period (FY)	2014-08-29 – 2016-03-31
Keywords	EEG
Outline of Annual Research Achievements	Initial injections of CNO did not significantly change EEG characteristics in AAV injected and control regions. The pattern of sleep stages also did not change. We repeated the experiments using larger AAV injections, but the results were the same. Measuring the signal correlation between the two recording sites. we found unusually high correlation during waking, NREM and REM sleep and concluded that our surface EEG signals were significantly contaminated by volume-conducted signals, mostly hippocampal theta activity. We changed our recording strategy to ensure that we were recording locally generated signals. We used fine wire electrodes in monopolar and bipolar configurations. Only bipolar electrodes showed truly local signals. Unfortunately, bipolar recordings require twice the number of amplifier connections, increasing the complexity of the surgery and electronics. We have now solved these problems and are repeating the experiments with CNO injections.
Research Progress Status	27年度が最終年度であるため、記入しない。
Strategy for Future Research Activity	27年度が最終年度であるため、記入しない。