2015 Fiscal Year Final Research Report
The homologous recombination reaction in chromatin
| Project/Area Number |
26890023
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| Research Category |
Grant-in-Aid for Research Activity start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Genome biology
|
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2014-08-29 – 2016-03-31
|
| Keywords | クロマチン / 相同組換え / リンカーヒストン / ヒストン修飾 / RAD51 / RAD54 |
|---|
| Outline of Final Research Achievements |
Homologous recombination functions in mitotic DNA double-strand break repair and meiotic genetic recombination. In eukaryotic nucleus, homologous recombination occurs in higher ordered chromatin. However, how the chromatin architecture involved in homologous recombination reaction has not been elucidated. In this study, we revealed that the testis-specific H1 variant H1T forms
less compacted chromatin and weakly inhibited the homologous recombination reaction as compared to the representative H1 variant. In addition, we clarify how the mono-ubiquitination of histone, which is required for DNA double-strand break repair, regulates the chromatin structure.
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| Free Research Field |
生化学
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