2015 Fiscal Year Final Research Report
Experimental evolution of an RNA enzyme using droplet-based microfluidics
| Project/Area Number |
26891009
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Evolutionary biology
|
|---|
| Research Institution | University of Toyama |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
Griffiths Andrew D. ESPCI ParisTech, 教授
|
|---|
| Project Period (FY) |
2014-08-29 – 2016-03-31
|
| Keywords | 人工進化 / RNA酵素 / マイクロ流体システム / エマルジョン |
|---|
| Outline of Final Research Achievements |
In this study, we focused on developing a compartmentalized ribozyme evolution system using microfluidic technology to pursue potential of catalytic RNAs. It is composed of two parts; “compartmentalized ribozyme expression system” and “droplet-based microfluidics”. First, we established the former by optimizing components of an RNA expression system we have previously developed. Second, the latter, an original system which is capable of multi-color fluorescence-based analysis and sorting of droplets, was constructed. Currently, InDrop evolution experiment of ribozymes is undergoing by using the system described above. The drop microfluidic system and devices developed in this study will contribute the basic technology for further artificial RNA evolution experiments.
|
| Free Research Field |
進化分子工学
|
