2015 Fiscal Year Final Research Report
Elucidation of the roles of cytoskeleton in plant tip growth
| Project/Area Number |
26891020
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Miyagi University |
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Principal Investigator |
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Keywords | 先端成長 / 微小管 / アクチン繊維 / コケ植物 / フォルミン / ライブイメージング |
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| Outline of Final Research Achievements |
Tip-growing cells display polarized cell growth. Microtubules (MTs) and actin filaments (AFs) have been implicated in regulating directionality and expansion. However, the regulatory interaction between MTs and AFs in tip-growing plant cells remains unclear. In this project, we showed that the proper generation of AFs was likely dependent on the existing MTs in the polarized expansion zone of caulonemal cells of the moss Physcomitrella patens. Moreover, the actin polymerizing factor formins, For2A and 2B, were involved in the regulation of the rate of cell growth. The live-imaging technique revealed that AF foci were generated in the existing MT foci in the expansion zone. The MT depolymerization led to the loss of the AF foci, suggesting that the AF foci are formed on the MT foci. The inducible RNAi screening of the putative MT-AF regulators indicated that For2A and 2B played roles on promoting the expansion rate.
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| Free Research Field |
植物細胞生理学
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