2015 Fiscal Year Final Research Report
Analysis of the mechanisms by which the extracellular dsRNA systemically spreads.
| Project/Area Number |
26891023
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Keywords | 全身性RNAi |
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| Outline of Final Research Achievements |
In C. elegans, dsRNA can systemically spread throughout the animal and lead to non-cell autonomous RNAi in cells distinct from the cells where dsRNA is originally introduced. This RNA silencing phenomenon is called the “systemic RNAi”. Systemic RNAi is thought to largely depend on vesicle transport because clathrin-dependent endocytosis and several proteins that localize endomembrane systems have been shown to be involved in systemic RNAi. However, underlying molecular mechanisms by which dsRNA is secreted to the extracellular space and enters into cells still unclear. To identify new factors that is involved in systemic dsRNA spread, genetic screening in which a strain defective in systemic RNAi restores RNAi ability was performed using C. elegans. One of the causal genes of the mutants was identified and analyzed.
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| Free Research Field |
生物学
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