Construction of experimental method for SRK-SCR interaction by using an SRK protein from plant cells.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26892004
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: Tohoku University
• Principal Investigator: Yamamoto Masaya 東北大学, (連合)農学研究科(研究院), 助教 (70732543)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 自家不和合性 / アブラナ科植物 / 分子細胞生物学 / ペプチド-リガンド相互作用

Outline of Final Research Achievements

The Brassicaceae plants possess the self-incompatible mechanism to avoid a self-fertilization. SRK, which is a receptor kinase in the stigma cell, and SCR, which is a pollen coat-localized ligand, play a major role in self-incompatible response. Although SRK-SCR interaction is important for self-incompatibility, its molecular mechanism remains unclear. Therefore, I made to construct the experimental method for analysis of SRK-SCR interaction. For this purpose, I developed preparation methods for SRK protein from A. thaliana cells and SCR protein from E. coli cells. By using SCR protein, we analyzed a BoSCR-45, and found an interested property. I would like to investigate the molecular mechanism of SRK-SCR interaction by using developed method in this work.

Free Research Field

分子細胞生物学