Analysis of novel AAA ATPase complex involved in endocytosis in Aspergillus oryzae

2015 Fiscal Year Final Research Report

• Project/Area Number: 26892022
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: Kyushu University
• Principal Investigator: Higuchi Yujiro 九州大学, (連合)農学研究科(研究院), 助教 (50732765)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: AAA ATPase / 麹菌 / エンドサイトーシス / AipA / エイソソーム / AoPil1

Outline of Final Research Achievements

AAA (ATPases associated with diverse cellular activities) ATPase localises at versatile intracellular cites and functions mainly in dissociation or degradation of protein complex. In koji mold Aspergillus oryzae, a AAA ATPase AipA had been identified as an interacting protein with an endocytic protein AoAbp1; however, molecular function of AipA had not been analysed in detail. In this study, it was revealed that AipA interacts with AoPil1, which is a putative component of eisosome, an endosomal structure at the plasma membrane. In addition, it was confirmed that AipA colocalises with AoPil1 in A. oryzae cells. At last, it was shown that aipA disruptant exhibits the sensitivity against inhibitors of ergosterol biosynthesis.

Free Research Field

応用分子細胞微生物学