2015 Fiscal Year Final Research Report
Expression analyzing of radiation responsive genes in human hematopoietic stem/progenitor cells
| Project/Area Number |
26893007
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Keywords | 造血幹細胞 / 放射線 / 網羅的遺伝子解析 / 放射線感受性 |
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| Outline of Final Research Achievements |
To clarify the nature of genes that contribute to the radiosensitivity of human hematopoietic stem/progenitor cells (HSPCs), we analyzed the gene expression profiles detected in HSPCs irradiated with 2 Gy X-rays after culture with or without an optimal combination of hematopoietic cytokines. A comprehensive genetic analysis to search for genes associated with cellular radiosensitivity was undertaken, and we found that the expressions of the downstream genes of MYC oncogene increased after X-irradiation. In fact, the activation of MYC was observed immediately after X-irradiation, and was the only gene still showing activation at 6 h after irradiation. Activated gene regulator function of MYC due to irradiation was suppressed by culturing the HSPCs with combinations of cytokines, which exerted radioprotective effects. MYC was strongly associated with the radiosensitivity of HSPCs.
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| Free Research Field |
放射線科学
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