2014 Fiscal Year Final Research Report
The roles of Sirt1 in the pancreas alpha cells
| Project/Area Number |
26893031
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Endocrinology
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
KIKUCHI Osamu 群馬大学, 生体調節研究所, 研究員 (40739009)
|
|---|
| Project Period (FY) |
2014-08-29 – 2015-03-31
|
| Keywords | グルカゴン / Sirt1 |
|---|
| Outline of Final Research Achievements |
We hypothesized that “Sirt1 up-regulates cell differentiation, proliferation and neogenesis, and proglucagon gene expression via activation of FoxO1 in α cells”. To investigate this hypothesis, we analyzed phenotype of the α cell-specific Sirt1 gene knockout (KO) mice.
The KO mice showed a significant suppression of recovery from insulin induced hypoglycemia compared with the control mice. This result suggests glucagon secretion was impaired in the KO mice. Moreover, we validated a Sirt1 inhibitor suppressed glucagon secretion and an activator enhanced it by in vitro analysis used α cell line. Both results supported the hypothesis.
|
| Free Research Field |
糖尿病
|
