2015 Fiscal Year Final Research Report
Comprehensive Analysis of Chromatin Structure in Hepatocellular Carcinoma Based on Somatic Mutation
| Project/Area Number |
26893045
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Keywords | 肝細胞癌 / FAIRE / エピゲノム / ゲノム |
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| Outline of Final Research Achievements |
In genesis and progression of tumor, not only genetic changes (gene mutations) but also alteration of systems that regulate genes are required. In this research, we focused on chromatin structure composed of DNA and proteins as one of the regulation systems of genes, and tried to identify genome wide chromatin structure changes through genesis and progression of cancer. We identified the genome wide chromatin structure using frozen surgical specimens of hepatocellular carcinoma with the FAIRE (Formaldehyde-Assisted Isolation of Regulatory Elements) method and a next generation sequencer, and compared the chromatin structure between hepatocellular carcinoma and background liver. By examining the chromatin structure changes based on the somatic mutations of oncogenes, we identified the regions of chromatin alteration specific to CTNNB1 mutation.
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| Free Research Field |
肝細胞癌，エピゲノム，ゲノム
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