The effect of ostecyte specific miRNA in response to mechanical stress

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893067
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Tohoku University (2015); Tokyo Medical and Dental University (2014)
• Principal Investigator: Shiwaku Yukari 東北大学, 歯学研究科(研究院), 助教 (80736190)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 骨細胞 / メカニカルストレス / マイクロRNA

Outline of Final Research Achievements

In this study, we focused on osteocyte specific miRNA and examined the effect of miRNA in osteocyte differentiation and response to mechanical stress. We isolated the osteocytes from osteocyte specific GFP expression mice by using FACS and identified miRNA expressed higher or lower than osteoblasts through the new generation sequencer. When some of miRNA was overexpressed in osteocyte, its differentiation was inhibited. Moreover, we identified the target genes of osteocyte specific miRNA by in silico analysis and examined protein expression and transcriptional activity of target genes. In conclusion, we succeeded to clarify one of the molecular mechanism by which miRNA regulates osteocyte differentiation.

Free Research Field

骨代謝