Elucidation of mechanism of the protection against allergies by LPS

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893089
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Tohoku University (2015); University of Toyama (2014)
• Principal Investigator: MIzuno Natsumi 東北大学, 薬学研究科(研究院), 助教 (40738621)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: LPS / 抗原提示細胞 / iNOS

Outline of Final Research Achievements

We examined mechanisms for the prevention of allergic reactions by infections from the point of view of the relation between LPS (lipopolysaccharide)-priming and antigen-uptake capacity of dendritic cells. First, we established an experimental model, in which LPS, as an endotoxin, protected mice against the subsequent development of allergic reactions. The accumulation of antigen up-taking cells in the lymph nodes in LPS-primed mice was significantly less than that in control mice. Interestingly, the age and term of LPS-priming were important for the prevention of lymph node-homing antigen. In addition, the uptake of antigen in bone marrow derived-dendritic cells (BMDCs) was lowered in LPS-primed mice than in control mice. The LPS-priming induced the expression of the inducible nitric oxide synthase (iNOS) and the highly NO-production in BMDCs. Furthermore, the iNOS inhibitor recovered the capacity of antigen-uptake in BMDCs from LPS-primed mice.

Free Research Field

医歯薬学