Molecular based analysis of HSP47 and novel therapies development in intestinal fibrosis of Crohn`s disease

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893122
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Kyoto University
• Principal Investigator: HONZAWA Yusuke 京都大学, 医学(系)研究科(研究院), 医員 (90737884)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: クローン病 / 炎症性腸疾患 / heat shock protein 47 / 腸管線維化

Outline of Final Research Achievements

To elucidate the mechanisms of intestinal fibrosis related to Crohn`s disease (CD), we focused heat shock protein (HSP) 47 associated with several fibrotic disease in human and mouse intestinal tissues samples and cell lines. Expressions of HSP47, collagen, IL-17A were elevated in intestinal tissues of patients with CD. Additionally, IL-17A enhanced HSP47 and collagen production in human myofibroblast cell line (CCD-18Co) and mouse fibroblast cell line (NIH3T3). IL-17A-induced HSP47 expression associated with JNK pathway in these cell lines. These results suggested that the mechanisms of IL-17A-induced HSP47 contributed to the pathology of intestinal fibrosis of CD.

Free Research Field

消化器内科