Association between intestinal inflammation and indigenous bacteria inhibit in Peyer's patch

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893136
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Osaka University
• Principal Investigator: Hiyama Satoshi 大阪大学, 医学(系)研究科(研究院), 寄附講座助教 (10735335)
• Co-Investigator(Kenkyū-buntansha): TUJII MASAHIKO 東大阪市立総合病院, 院長 (40303937) ; IIJIMA HIDEKI 大阪大学, 医学系研究科, 講師 (90444520) ; SHINZAKI SHINICHIRO 大阪大学, 医学系研究科, 助教 (60546860)
• Co-Investigator(Renkei-kenkyūsha): IIDA TETUYA 大阪大学, 微生物病研究所, 教授 (90221746)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: パイエル板 / 腸管免疫

Outline of Final Research Achievements

Lymphoid tissue was resected from Peyer’s patches (PP) of the wild-type mice using laser microdissection method (LMD). When we analyzed the number of bacteria from the extracted DNA from PP and heart (negative control) using LMD, the number of bacteria involved in PP had higher tendency compared with those from heart. This indicates that detectable number of bacteria inhabits inside of PP.
Moreover, the profiles of bacteria identified from PP, heart and feces samples was evaluated using metagenomics analysis, which revealed that each group showed distinct profiles. Although potential contamination cannot be denied completely, this data suggests the possibility that specific bacteria species might exist inside of PP.

Free Research Field

消化器病学