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2015 Fiscal Year Final Research Report

Analysis of osteoblast differentiation mechanism using a Id2-deficient mice derived from iPS cells

Research Project

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Project/Area Number 26893143
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Dental engineering/Regenerative dentistry
Research InstitutionOsaka University

Principal Investigator

Uraguchi Shinya  大阪大学, 歯学部附属病院, 医員 (80737528)

Project Period (FY) 2014-08-29 – 2016-03-31
KeywordsiPS細胞 / 骨芽細胞分化 / Id2
Outline of Final Research Achievements

iPS cells are expected to be useful for alveolar bone augmentation/regeneration in dental implant and prothodontic treatment; however the mechanisms of iPS cell osteogenesis remain unclear. The objective is to investigate the role of Id2 in iPS cell osteogenesis by establishing Id2-deficient model iPS cells. The induction of iPS cells from gingival fibroblasts of Id2-/- or Id2+/+ mice was performed by retroviral transduction. iPS cells induced osteogenic differentiation and analyzed by ALP staining etc. The Id2-/--iPS cells showed higher osteogenic gene expression than Id2+/+-iPS cells. We established an osteogenesis model with Id2-deficient iPS cells, which demonstrated enhanced osteogenic differentiation and represent an important step toward the further analysis of novel mechanisms underlying iPS cell osteogenesis.

Free Research Field

歯科補綴学

URL: 

Published: 2017-05-10  

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