2015 Fiscal Year Final Research Report
Analysis of osteoblast differentiation mechanism using a Id2-deficient mice derived from iPS cells
Project/Area Number |
26893143
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Dental engineering/Regenerative dentistry
|
Research Institution | Osaka University |
Principal Investigator |
|
Project Period (FY) |
2014-08-29 – 2016-03-31
|
Keywords | iPS細胞 / 骨芽細胞分化 / Id2 |
Outline of Final Research Achievements |
iPS cells are expected to be useful for alveolar bone augmentation/regeneration in dental implant and prothodontic treatment; however the mechanisms of iPS cell osteogenesis remain unclear. The objective is to investigate the role of Id2 in iPS cell osteogenesis by establishing Id2-deficient model iPS cells. The induction of iPS cells from gingival fibroblasts of Id2-/- or Id2+/+ mice was performed by retroviral transduction. iPS cells induced osteogenic differentiation and analyzed by ALP staining etc. The Id2-/--iPS cells showed higher osteogenic gene expression than Id2+/+-iPS cells. We established an osteogenesis model with Id2-deficient iPS cells, which demonstrated enhanced osteogenic differentiation and represent an important step toward the further analysis of novel mechanisms underlying iPS cell osteogenesis.
|
Free Research Field |
歯科補綴学
|