The pharmacokinetic model development observing drug metabolism with the drug membrane transport using the human artificial chromosome.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893153
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Drug development chemistry
• Research Institution: Tottori University
• Principal Investigator: SATOH Daisuke 鳥取大学, 染色体工学研究センター, プロジェクト研究員 (40734992)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 薬物代謝酵素 / CYP / シトクロムP450 / 薬物動態 / 薬物代謝 / 肝細胞 / HepG2

Outline of Final Research Achievements

In this study, it was intended that I made evaluable gastrointestinal models for drug metabolism and the drug absorption at the same time. The method carried the purpose genes in the artificial chromosome which our laboratory developed and aimed at carrying it into gastrointestinal cells (Caco-2 cells). By this development, I built an artificial chromosome for CYPs overexpression-Caco-2 cells. Furthermore, the artificial chromosome which I built showed what I could put into a cells (HT1080 cells) derived from different human cells. Furthermore, this artificial chromosome held transgene in an HT1080 cells and showed that I had protein expression. It will be thought that I can achieve this purpose by carring the artificial chromosome which I developed in this study into Caco-2 cells in future.

Free Research Field

薬物動態学